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Anti-ATP2B1 Rabbit Monoclonal Antibody #3355

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Flow cytometric analysis of ATP2B1 expression in HepG2 cells using anti-ATP2B1 antibody (Cat#3355, 1:2,000). Green, isotype control; red, ATP2B1.

Immunocytochemical staining of HepG2 cells with anti-ATP2B1 antibody (Cat#3355, 1:1,000). Nuclei were stained blue with DAPI; ATP2B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-ATP2B1 antibody (Cat#3355). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP2B1 antibody (Cat#3355, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

ATP2B1
货号：

3355
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-ATP2B1 Rabbit Monoclonal Antibody
Aliases	ATP2B1; ATPase Plasma Membrane Ca2+ Transporting 1; PMCA1; Plasma Membrane Calcium-Transporting ATPase 1; ATPase, Ca++ Transporting, Plasma Membrane 1; Plasma Membrane Calcium ATPase Isoform 1; Plasma Membrane Calcium Pump Isoform 1; Plasma Membrane Calcium Pump; EC 7.2.2.10; EC 3.6.3.8; EC 3.6.3; PMCA1kb; MRD66
Background	Gene Name: ATP2B1 NCBI Gene Entry: 490 UniProt Entry: P20020
Application Information	Molecular Weight: Predicted, 135 kDa; observed, 130-250 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB5270 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human PMCA1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of ATP2B1 expression in HepG2 cells using anti-ATP2B1 antibody (Cat#3355, 1:2,000). Green, isotype control; red, ATP2B1.
Immunocytochemical staining of HepG2 cells with anti-ATP2B1 antibody (Cat#3355, 1:1,000). Nuclei were stained blue with DAPI; ATP2B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-ATP2B1 antibody (Cat#3355). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP2B1 antibody (Cat#3355, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

ATP2B1
货号：

3355
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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