Flow cytometric analysis of FMR1 expression in C2C12 cells using anti-FMR1 antibody (Cat#3365, 1:2,000). Green, isotype control; red, FMR1.
Immunocytochemical staining of C2C12 cells with anti-FMR1 antibody (Cat#3365, 1:1,000) . Nuclei were stained blue with DAPI; FMR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-FMR1 antibody (Cat#3365). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FMR1 antibody (Cat#3365, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). FMR1, fragile X messenger ribonucleoprotein 1.
Flow cytometric analysis of FMR1 expression in C2C12 cells using anti-FMR1 antibody (Cat#3365, 1:2,000). Green, isotype control; red, FMR1.
Immunocytochemical staining of C2C12 cells with anti-FMR1 antibody (Cat#3365, 1:1,000) . Nuclei were stained blue with DAPI; FMR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-FMR1 antibody (Cat#3365). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FMR1 antibody (Cat#3365, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). FMR1, fragile X messenger ribonucleoprotein 1.
Flow cytometric analysis of FMR1 expression in C2C12 cells using anti-FMR1 antibody (Cat#3365, 1:2,000). Green, isotype control; red, FMR1.
Immunocytochemical staining of C2C12 cells with anti-FMR1 antibody (Cat#3365, 1:1,000) . Nuclei were stained blue with DAPI; FMR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-FMR1 antibody (Cat#3365). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FMR1 antibody (Cat#3365, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). FMR1, fragile X messenger ribonucleoprotein 1.
