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Anti-FTO Rabbit Monoclonal Antibody #3379

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Flow cytometric analysis of FTO expression in HepG2 cells using anti-FTO antibody (Cat#3379, 1:2,000). Green, isotype control; red, FTO.

Immunocytochemical staining of HepG2 cells with anti-FTO antibody (Cat#3379, 1:1,000). Nuclei were stained blue with DAPI; FTO was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.

Western blotting analysis using anti-FTO antibody (Cat#3379). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FTO antibody (Cat#3379, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

FTO
货号：

3379
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-FTO Rabbit Monoclonal Antibody
Aliases	FTO; FTO Alpha-Ketoglutarate Dependent Dioxygenase; KIAA1752; ALKBH9; U6 Small Nuclear RNA (2'-O-Methyladenosine-N(6)-)-Demethylase FTO; U6 Small Nuclear RNA N(6)-Methyladenosine-Demethylase FTO; MRNA (2'-O-Methyladenosine-N(6)-)-Demethylase FTO; Alpha-Ketoglutarate-Dependent Dioxygenase FTO; Intragenic FTO Exon 9 Containing Transcript; MRNA N(6)-Methyladenosine Demethylase FTO; Fat Mass And Obesity-Associated Protein; TRNA N1-Methyl Adenine Demethylase FTO; Fat Mass And Obesity Associated; M6A(M)-Demethylase FTO; AlkB Homolog 9; MGC5149; IFEX9; FTO, Alpha-Ketoglutarate; Dependent Dioxygenase; Alpha-Ketoglutarate-Dependent Dioxygenase; EC 1.14.11.53; EC 1.14.11.-; BMIQ14; GDFD
Background	Gene Name: FTO NCBI Gene Entry: 79068 UniProt Entry: Q9C0B1
Application Information	Molecular Weight: Predicted, 58 kDa; observed, 58 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB5395 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human FTO
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of FTO expression in HepG2 cells using anti-FTO antibody (Cat#3379, 1:2,000). Green, isotype control; red, FTO.
Immunocytochemical staining of HepG2 cells with anti-FTO antibody (Cat#3379, 1:1,000). Nuclei were stained blue with DAPI; FTO was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-FTO antibody (Cat#3379). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FTO antibody (Cat#3379, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

FTO
货号：

3379
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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