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Anti-Calpain 1 Rabbit Monoclonal Antibody #4328

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Flow cytometric analysis of calpain 1 expression in C2C12 cells using anti-calpain 1 antibody (Cat#4328, 1:2,000). Green, isotype control; red, calpain 1.
Immunocytochemical staining of C2C12 cells with anti-Calpain 1 antibody (Cat#4328, 1:1,000) . Nuclei were stained blue with DAPI; Calpain 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-calpain 1 antibody (Cat#4328). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-calpain 1 antibody (Cat#4328, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    CAPN1
  • 货号：
    4328
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-Calpain 1 Rabbit Monoclonal Antibody
Aliases CAPN1; Calpain 1; CANPL1; MuCANP; CANP; Cell Proliferation-Inducing Gene 30 Protein; Calcium-Activated Neutral Proteinase 1; Calpain 1, (Mu/I) Large Subunit; Calpain-1 Catalytic Subunit; Calpain-1 Large Subunit; Micromolar-Calpain; Calpain Mu-Type; EC 3.4.22.52; CANP; MuCL; Cell Proliferation-Inducing Protein 30; Calpain, Large Polypeptide L1; EC 3.4.22; MUCANP; CANP1; SPG76; MUCL
Background

Gene Name: CAPN1

NCBI Gene Entry: 823

UniProt Entry: P07384
Application Information

Molecular Weight: Predicted, 82 kDa; observed, 75 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10670

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Calpain 1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of calpain 1 expression in C2C12 cells using anti-calpain 1 antibody (Cat#4328, 1:2,000). Green, isotype control; red, calpain 1.
  • Immunocytochemical staining of C2C12 cells with anti-Calpain 1 antibody (Cat#4328, 1:1,000) . Nuclei were stained blue with DAPI; Calpain 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-calpain 1 antibody (Cat#4328). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-calpain 1 antibody (Cat#4328, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    CAPN1
  • 货号：
    4328
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of calpain 1 expression in C2C12 cells using anti-calpain 1 antibody (Cat#4328, 1:2,000). Green, isotype control; red, calpain 1.
Immunocytochemical staining of C2C12 cells with anti-Calpain 1 antibody (Cat#4328, 1:1,000) . Nuclei were stained blue with DAPI; Calpain 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-calpain 1 antibody (Cat#4328). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-calpain 1 antibody (Cat#4328, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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