Flow cytometric analysis of vimentin expression in HepG2 cells using anti-vimentin antibody (Cat#3471, 1:2,000). Green, isotype control; red, vimentin.
Immunocytochemical staining of HepG2 cells with anti-Vimentin antibody (Cat#3471, 1:1,000) . Nuclei were stained blue with DAPI; Vimentin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human endometrial carcinoma using anti-vimentin antibody (Cat#3471, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-vimentin antibody (Cat#3471). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-vimentin antibody (Cat#3471, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
