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Anti-DNAJB1 Mouse Monoclonal Antibody #3520

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Flow cytometric analysis of DNAJB1 expression in HepG2 cells using anti-DNAJB1 antibody (Cat#3520, 1:2,000). Green, isotype control; red, DNAJB1.

Immunocytochemical staining of HepG2 cells with anti-DNAJB1 antibody (Cat#3520, 1:1,000) . Nuclei were stained blue with DAPI; DNAJB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.

Western blotting analysis using anti-DNAJB1 antibody (Cat#3520). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNAJB1 antibody (Cat#3520, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

DNAJB1
货号：

3520
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-DNAJB1 Mouse Monoclonal Antibody
Aliases	DNAJB1; DnaJ Heat Shock Protein Family (Hsp40) Member B1; RSPH16B; Hsp40; HSPF1; Sis1; DnaJ (Hsp40) Homolog, Subfamily B, Member 1; DnaJ Homolog Subfamily B Member 1; Heat Shock 40 KDa Protein 1; DnaJ Protein Homolog 1; Human DnaJ Protein 1; Radial Spoke 16 Homolog B (Chlamydomonas); Radial Spoke 16 Homolog B; Heat Shock Protein 40; DNAJ1; HSP40; HDj-1; Hdj1; HDJ1
Background	Gene Name: DNAJB1 NCBI Gene Entry: 3337 UniProt Entry: P25685
Application Information	Molecular Weight: Predicted, 38 kDa; observed, 38 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB6325 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human DNAJB1
Isotype	Mouse IgG2a
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:2,500-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of DNAJB1 expression in HepG2 cells using anti-DNAJB1 antibody (Cat#3520, 1:2,000). Green, isotype control; red, DNAJB1.
Immunocytochemical staining of HepG2 cells with anti-DNAJB1 antibody (Cat#3520, 1:1,000) . Nuclei were stained blue with DAPI; DNAJB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.
Western blotting analysis using anti-DNAJB1 antibody (Cat#3520). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNAJB1 antibody (Cat#3520, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

DNAJB1
货号：

3520
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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