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Anti-RAMP1 Rabbit Monoclonal Antibody #3687

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Flow cytometric analysis of Receptor activity modifying protein 1 expression in HepG2 cells using anti-Receptor activity modifying protein 1 antibody (Cat#3687, 1:2,000). Green, isotype control; red, Receptor activity modifying protein 1.
Immunocytochemical staining of HepG2 cells with anti-Receptor activity modifying protein 1 antibody (Cat#3687, 1:1,000) . Nuclei were stained blue with DAPI; Receptor activity modifying protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-receptor activity modifying protein 1 antibody (Cat#3687). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-receptor activity modifying protein 1 antibody (Cat#3687, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    RAMP1
  • 货号:
    3687
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-RAMP1 Rabbit Monoclonal Antibody
Aliases RAMP1; Receptor Activity Modifying Protein 1; Receptor Activity-Modifying Protein 1; CRLR Activity-Modifying Protein 1; Calcitonin Receptor-Like Receptor Activity Modifying Protein 1; Calcitonin-Receptor-Like Receptor Activity-Modifying Protein 1; Receptor (G Protein-Coupled) Activity Modifying Protein 1; Receptor (Calcitonin) Activity Modifying Protein 1
Background

Gene Name: RAMP1

NCBI Gene Entry: 10267

UniProt Entry: O60894

Application Information

Molecular Weight: Predicted, 17 kDa; observed, 17 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB6910

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human RAMP1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Receptor activity modifying protein 1 expression in HepG2 cells using anti-Receptor activity modifying protein 1 antibody (Cat#3687, 1:2,000). Green, isotype control; red, Receptor activity modifying protein 1.
  • Immunocytochemical staining of HepG2 cells with anti-Receptor activity modifying protein 1 antibody (Cat#3687, 1:1,000) . Nuclei were stained blue with DAPI; Receptor activity modifying protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
  • Western blotting analysis using anti-receptor activity modifying protein 1 antibody (Cat#3687). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-receptor activity modifying protein 1 antibody (Cat#3687, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of Receptor activity modifying protein 1 expression in HepG2 cells using anti-Receptor activity modifying protein 1 antibody (Cat#3687, 1:2,000). Green, isotype control; red, Receptor activity modifying protein 1.
Immunocytochemical staining of HepG2 cells with anti-Receptor activity modifying protein 1 antibody (Cat#3687, 1:1,000) . Nuclei were stained blue with DAPI; Receptor activity modifying protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-receptor activity modifying protein 1 antibody (Cat#3687). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-receptor activity modifying protein 1 antibody (Cat#3687, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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