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Anti-ALDH2 Mouse Monoclonal Antibody #3703

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Flow cytometric analysis of Aldehyde dehydrogenase 2 family member expression in HepG2 cells using anti-Aldehyde dehydrogenase 2 family member antibody (Cat#3703, 1:2,000). Green, isotype control; red, Aldehyde dehydrogenase 2 family member.
Immunocytochemical staining of C2C12 cells with anti-Aldehyde dehydrogenase 2 family member antibody (Cat#3703, 1:1,000) . Nuclei were stained blue with DAPI;Aldehyde dehydrogenase 2 family member was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-aldehyde dehydrogenase 2 family member antibody (Cat#3703). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-aldehyde dehydrogenase 2 family member antibody (Cat#3703, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    ALDH2
  • 货号:
    3703
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-ALDH2 Mouse Monoclonal Antibody
Aliases ALDH2; Aldehyde Dehydrogenase 2 Family Member; Aldehyde Dehydrogenase 2 Family (Mitochondrial); Aldehyde Dehydrogenase, Mitochondrial; ALDH Class 2; EC 1.2.1.3; ALDH-E2; ALDHI; ALDM; Nucleus-Encoded Mitochondrial Aldehyde Dehydrogenase 2; Epididymis Secretory Sperm Binding Protein; Acetaldehyde Dehydrogenase 2; Liver Mitochondrial ALDH; EC 1.2.1
Background

Gene Name: ALDH2

NCBI Gene Entry: 217

UniProt Entry: P05091

Application Information

Molecular Weight: Predicted, 56 kDa; observed, 55 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB6990

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen Recombinant protein of human ALDH2
Isotype Mouse IgG1 kappa
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,500-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Aldehyde dehydrogenase 2 family member expression in HepG2 cells using anti-Aldehyde dehydrogenase 2 family member antibody (Cat#3703, 1:2,000). Green, isotype control; red, Aldehyde dehydrogenase 2 family member.
  • Immunocytochemical staining of C2C12 cells with anti-Aldehyde dehydrogenase 2 family member antibody (Cat#3703, 1:1,000) . Nuclei were stained blue with DAPI;Aldehyde dehydrogenase 2 family member was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
  • Western blotting analysis using anti-aldehyde dehydrogenase 2 family member antibody (Cat#3703). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-aldehyde dehydrogenase 2 family member antibody (Cat#3703, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of Aldehyde dehydrogenase 2 family member expression in HepG2 cells using anti-Aldehyde dehydrogenase 2 family member antibody (Cat#3703, 1:2,000). Green, isotype control; red, Aldehyde dehydrogenase 2 family member.
Immunocytochemical staining of C2C12 cells with anti-Aldehyde dehydrogenase 2 family member antibody (Cat#3703, 1:1,000) . Nuclei were stained blue with DAPI;Aldehyde dehydrogenase 2 family member was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-aldehyde dehydrogenase 2 family member antibody (Cat#3703). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-aldehyde dehydrogenase 2 family member antibody (Cat#3703, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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