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Anti-CAMK4 Rabbit Monoclonal Antibody #8154

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Flow cytometric analysis of CAMK4 expression in HepG2 cells using CAMK4 antibody (Cat#8154, 1:2,000). Green, isotype control; red, CAMK4.
Immunocytochemical staining of HepG2 cells with CAMK4 antibody (Cat#8154, 1:1,000). Nuclei were stained blue with DAPI; CAMK4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-CAMK4 antibody (Cat#8154). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CAMK4 antibody (Cat#8154, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). CAMK4, calcium/calmodulin dependent protein kinase IV.
  • 基因名:
    CAMK4
  • 货号:
    8154
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-CAMK4 Rabbit Monoclonal Antibody
Aliases Calcium/Calmodulin Dependent Protein Kinase IV; CaMK-GR; CaMKIV; Calcium/Calmodulin-Dependent Protein Kinase Type IV Catalytic Chain; Brain Ca++-Calmodulin-Dependent Protein Kinase Type IV; Calcium/Calmodulin-Dependent Protein Kinase Type IV; CAM Kinase- GR; CAM Kinase IV; CaMK IV; Brain Ca(2+)-Calmodulin-Dependent Protein Kinase Type IV; CaM Kinase-GR; EC 2.7.11.17; EC 2.7.11; CAMK-GR; CAMKIV; CaMK; CAMK
Background

Gene Name: CAMK4

NCBI Gene Entry: 814

UniProt Entry: Q16566

Application Information

Molecular Weight: Predicted, 52 kDa; observed, 52 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1900

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human CAMKIV
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of CAMK4 expression in HepG2 cells using CAMK4 antibody (Cat#8154, 1:2,000). Green, isotype control; red, CAMK4.
  • Immunocytochemical staining of HepG2 cells with CAMK4 antibody (Cat#8154, 1:1,000). Nuclei were stained blue with DAPI; CAMK4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-CAMK4 antibody (Cat#8154). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CAMK4 antibody (Cat#8154, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). CAMK4, calcium/calmodulin dependent protein kinase IV.
Citation(0)
Flow cytometric analysis of CAMK4 expression in HepG2 cells using CAMK4 antibody (Cat#8154, 1:2,000). Green, isotype control; red, CAMK4.
Immunocytochemical staining of HepG2 cells with CAMK4 antibody (Cat#8154, 1:1,000). Nuclei were stained blue with DAPI; CAMK4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-CAMK4 antibody (Cat#8154). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CAMK4 antibody (Cat#8154, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). CAMK4, calcium/calmodulin dependent protein kinase IV.
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