Anti-MDM2 Proto-Oncogene Mouse Monoclonal Antibody - 产品中心

Anti-MDM2 Proto-Oncogene Mouse Monoclonal Antibody #3716

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Flow cytometric analysis of MDM2 expression in C2C12 cells using anti-MDM2 antibody (Cat#3716, 1:2,000). Green, isotype control; red, MDM2.

Immunocytochemical staining of C2C12 cells with anti-MDM2 antibody (Cat#3716, 1:1,000) . Nuclei were stained blue with DAPI;MDM2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-MDM2 antibody (Cat#3716). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MDM2 antibody (Cat#3716, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

MDM2
货号：

3716
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-MDM2 Proto-Oncogene Mouse Monoclonal Antibody
Aliases	MDM2; MDM2 Proto-Oncogene; HDM2; MDM2 Proto-Oncogene, E3 Ubiquitin Protein Ligase; E3 Ubiquitin-Protein Ligase Mdm2; Oncoprotein Mdm2; MGC5370; Mdm2, Transformed 3T3 Cell Double Minute 2, P53 Binding Protein (Mouse); Mdm2, Transformed 3T3 Cell Double Minute 2, P53 Binding Protein; Mouse Double Minute 2, Human Homolog Of; P53-Binding Protein; Double Minute 2, Human Homolog Of; P53-Binding Protein; Mdm2, P53 E3 Ubiquitin Protein Ligase Homolog; MDM2 Oncogene, E3 Ubiquitin Protein Ligase; Mdm2 P53 Binding Protein Homolog (Mouse); RING-Type E3 Ubiquitin Transferase Mdm2; P53-Binding Protein Mdm2; Double Minute 2 Protein; EC 2.3.2.27; EC 6.3.2; ACTFS; HDMX; LSKB; Hdm2
Background	Gene Name: MDM2 NCBI Gene Entry: 4193 UniProt Entry: Q00987
Application Information	Molecular Weight: Predicted, 55 kDa; observed, 60 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB7055 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human MDM2
Isotype	Mouse IgG1 kappa
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:2,500-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of MDM2 expression in C2C12 cells using anti-MDM2 antibody (Cat#3716, 1:2,000). Green, isotype control; red, MDM2.
Immunocytochemical staining of C2C12 cells with anti-MDM2 antibody (Cat#3716, 1:1,000) . Nuclei were stained blue with DAPI;MDM2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-MDM2 antibody (Cat#3716). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MDM2 antibody (Cat#3716, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

MDM2
货号：

3716
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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