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Anti-MKI67 Mouse Monoclonal Antibody #3740

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Flow cytometric analysis of Marker of proliferation Ki-67 expression in HAP-1 cells using anti-Marker of proliferation Ki-67 antibody (Cat#3740, 1:2,000). Green, isotype control; red, Marker of proliferation Ki-67.
Immunocytochemical staining of HAP-1 cells with anti-Marker of proliferation Ki-67 antibody (Cat#3740, 1:1,000) . Nuclei were stained blue with DAPI; Marker of proliferation Ki-67 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：High. Scale bar, 20 μm.
Western blotting analysis using anti-marker of proliferation Ki-67 antibody (Cat#3740). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-marker of proliferation Ki-67 antibody (Cat#3740, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human gastric carcinoma using anti-marker of proliferation Ki-67 antibody (Cat#3740, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • 基因名：
    MKI67
  • 货号：
    3740
  • 应用：
    WB, FCM, IC, IHC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-MKI67 Mouse Monoclonal Antibody
Aliases MKI67; Marker Of Proliferation Ki-67; Antigen Identified By Monoclonal Antibody Ki-67; PPP1R105; MIB-1; Protein Phosphatase 1, Regulatory Subunit 105; Molecular Immunology Borstel Antibody 1; Proliferation Marker Protein Ki-67; Antigen Ki67; Ki-67; Proliferation-Related Ki-67 Antigen; Antigen KI-67; MIB-; KIA
Background

Gene Name: MKI67

NCBI Gene Entry: 4288

UniProt Entry: P46013
Application Information

Molecular Weight: Predicted, 359 kDa; observed, 359 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB7155

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC), immunohistochemistry (IHC)
Immunogen A synthesized peptide derived from human Ki67
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,500-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Immunohistochemistry (IHC): 1:50-1:100
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Marker of proliferation Ki-67 expression in HAP-1 cells using anti-Marker of proliferation Ki-67 antibody (Cat#3740, 1:2,000). Green, isotype control; red, Marker of proliferation Ki-67.
  • Immunocytochemical staining of HAP-1 cells with anti-Marker of proliferation Ki-67 antibody (Cat#3740, 1:1,000) . Nuclei were stained blue with DAPI; Marker of proliferation Ki-67 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：High. Scale bar, 20 μm.
  • Western blotting analysis using anti-marker of proliferation Ki-67 antibody (Cat#3740). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-marker of proliferation Ki-67 antibody (Cat#3740, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human gastric carcinoma using anti-marker of proliferation Ki-67 antibody (Cat#3740, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Citation(0)
  • 基因名：
    MKI67
  • 货号：
    3740
  • 应用：
    WB, FCM, IC, IHC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of Marker of proliferation Ki-67 expression in HAP-1 cells using anti-Marker of proliferation Ki-67 antibody (Cat#3740, 1:2,000). Green, isotype control; red, Marker of proliferation Ki-67.
Immunocytochemical staining of HAP-1 cells with anti-Marker of proliferation Ki-67 antibody (Cat#3740, 1:1,000) . Nuclei were stained blue with DAPI; Marker of proliferation Ki-67 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：High. Scale bar, 20 μm.
Western blotting analysis using anti-marker of proliferation Ki-67 antibody (Cat#3740). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-marker of proliferation Ki-67 antibody (Cat#3740, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human gastric carcinoma using anti-marker of proliferation Ki-67 antibody (Cat#3740, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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