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Anti-beta Tubulin Rabbit Monoclonal Antibody #3792

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Western blotting analysis using anti-β-Tubulin antibody (Cat#3792). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-β-Tubulin antibody (Cat#3792, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Flow cytometric analysis of β-Tubulin expression in HAP-1 cells using anti-β-Tubulin antibody (Cat#3792, 1:2,000). Green, isotype control; red, β-Tubulin.

Immunocytochemical staining of HAP1 cells with anti-β-Tubulin antibody (Cat#3792, 1:1,000). Nuclei were stained blue with DAPI; β-Tubulin stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm. Permeabilization: Methanol.

基因名：

TUBB
货号：

3792
应用：

WB
物种反应性：

H, M, R

规格：

50 μL，100 μL

价格：

¥ 600.00，¥ 1,000.00

Information

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Information

Product Name	Anti-beta Tubulin Rabbit Monoclonal Antibody
Aliases	TUBB; Tubulin Beta Class I; OK/SW-Cl.56; Tubb5; M40; Tubulin, Beta Polypeptide; Tubulin Beta-5 Chain; Tubulin Beta Chain; MGC16435; Epididymis Secretory Sperm Binding Protein; Tubulin, Beta Class I; Class I Beta-Tubulin; Tubulin Beta-1 Chain; Beta Ib Tubulin; Beta1-Tubulin; Tubulin, Beta; CDCBM6; CSCSC1; TUBB1; TUBB5
Background	Gene Name: TUBB NCBI Gene Entry: 203068 UniProt Entry: P07437
Application Information	Molecular Weight: Predicted, 50 kDa; observed, 50 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB7895 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human beta Tubulin
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:2,000-1:10,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Western blotting analysis using anti-β-Tubulin antibody (Cat#3792). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-β-Tubulin antibody (Cat#3792, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of β-Tubulin expression in HAP-1 cells using anti-β-Tubulin antibody (Cat#3792, 1:2,000). Green, isotype control; red, β-Tubulin.
Immunocytochemical staining of HAP1 cells with anti-β-Tubulin antibody (Cat#3792, 1:1,000). Nuclei were stained blue with DAPI; β-Tubulin stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm. Permeabilization: Methanol.

Citation(0)

基因名：

TUBB
货号：

3792
应用：

WB
物种反应性：

H, M, R

规格：

50 μL，100 μL

价格：

¥ 600.00，¥ 1,000.00

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