• 一抗
  • 二抗
  • 慢病毒
  • 细胞系
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Anti-beta Actin Mouse Monoclonal Antibody, HRP Conjugated #3793-HRP

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Western blotting analysis using anti-β-Actin (HRP Conjugated) antibody (Cat#3793-HRP, 1:10,000). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of β-Actin expression in H9c2 cells using anti-β-Actin antibody (Cat#3793-HRP, 1:2,000). Green, isotype control; red, β-Actin.
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using anti-β-actin (HRP Conjugated) antibody (Cat#3793-HRP, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • 基因名:
    ACTB
  • 货号:
    3793-HRP
  • 应用:
    WB, FCM, IHC
  • 物种反应性:
    H, M, R
规格:
50 μL,100 μL
价格:
¥ 600.00,¥ 1,000.00
Information
Product Name Anti-beta Actin Mouse Monoclonal Antibody, HRP Conjugated
Aliases ACTB; Actin Beta; Actin, Cytoplasmic 1; PS1TP5-Binding Protein 1; Beta Cytoskeletal Actin; I(2)-Actin; Beta-Actin; EC 3.6.4.-; PS1TP5BP1; Β-Actin; BKRNS; BRWS1; CSMH; DDS1; THC8; BNS
Background

Gene Name: ACTB

NCBI Gene Entry: 60

UniProt Entry: P60709

Application Information

Molecular Weight: Predicted, 42 kDa; observed, 42 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB7900

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry (IHC)

Immunogen A synthesized peptide derived from human beta Actin (HRP)
Isotype Mouse IgG2b
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:2,000
Immunohistochemistry (IHC): 1:50-1:100
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-β-Actin (HRP Conjugated) antibody (Cat#3793-HRP, 1:10,000). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of β-Actin expression in H9c2 cells using anti-β-Actin antibody (Cat#3793-HRP, 1:2,000). Green, isotype control; red, β-Actin.
  • Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using anti-β-actin (HRP Conjugated) antibody (Cat#3793-HRP, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Citation(0)
Western blotting analysis using anti-β-Actin (HRP Conjugated) antibody (Cat#3793-HRP, 1:10,000). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of β-Actin expression in H9c2 cells using anti-β-Actin antibody (Cat#3793-HRP, 1:2,000). Green, isotype control; red, β-Actin.
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using anti-β-actin (HRP Conjugated) antibody (Cat#3793-HRP, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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