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KD-Validated Anti-EIF4EBP1 Rabbit Monoclonal Antibody #63184

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Flow cytometric analysis of EIF4EBP1 expression in C2C12 cells using anti-EIF4EBP1 antibody (Cat#63184, 1:2,000). Green, isotype control; red, EIF4EBP1.
Immunocytochemical staining of C2C12 cells with anti-EIF4EBP1 antibody (Cat#63184, 1:1,000) . Nuclei were stained blue with DAPI; EIF4EBP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-EIF4EBP1 antibody (Cat#63184). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF4EBP1 antibody (Cat#63184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). EIF4EBP1, eukaryotic translation initiation factor 4E binding protein 1.
Western blotting analysis using anti-EIF4EBP1 antibody (Cat#63184). EIF4EBP1 expression in wild-type (WT) and EIF4EBP1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-EIF4EBP1 antibody (Cat#63184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using anti-EIF4EBP1 antibody (Cat#63184, 1:1,000), Top panel: wild-type (WT); Bottom panal: EIF4EBP1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; EIF4EBP1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
  • 基因名：
    EIF4EBP1
  • 货号：
    63184
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-EIF4EBP1 Rabbit Monoclonal Antibody
Aliases EIF4EBP1; Eukaryotic Translation Initiation Factor 4E Binding Protein 1; PHAS-I ; 4E-BP1; Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1; Eukaryotic Translation Initiation Factor 4E-Binding Protein 1; EIF4E-Binding Protein 1; 4EBP1; BP-1
Background

Gene Name: EIF4EBP1

NCBI Gene Entry: 1978

UniProt Entry: Q13541
Application Information

Molecular Weight: Predicted, 13 kDa, observed, 15-20 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4410

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human eIF4EBP1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of EIF4EBP1 expression in C2C12 cells using anti-EIF4EBP1 antibody (Cat#63184, 1:2,000). Green, isotype control; red, EIF4EBP1.
  • Immunocytochemical staining of C2C12 cells with anti-EIF4EBP1 antibody (Cat#63184, 1:1,000) . Nuclei were stained blue with DAPI; EIF4EBP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-EIF4EBP1 antibody (Cat#63184). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF4EBP1 antibody (Cat#63184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). EIF4EBP1, eukaryotic translation initiation factor 4E binding protein 1.
  • Western blotting analysis using anti-EIF4EBP1 antibody (Cat#63184). EIF4EBP1 expression in wild-type (WT) and EIF4EBP1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-EIF4EBP1 antibody (Cat#63184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HeLa cells using anti-EIF4EBP1 antibody (Cat#63184, 1:1,000), Top panel: wild-type (WT); Bottom panal: EIF4EBP1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; EIF4EBP1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Citation(0)
  • 基因名：
    EIF4EBP1
  • 货号：
    63184
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of EIF4EBP1 expression in C2C12 cells using anti-EIF4EBP1 antibody (Cat#63184, 1:2,000). Green, isotype control; red, EIF4EBP1.
Immunocytochemical staining of C2C12 cells with anti-EIF4EBP1 antibody (Cat#63184, 1:1,000) . Nuclei were stained blue with DAPI; EIF4EBP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-EIF4EBP1 antibody (Cat#63184). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF4EBP1 antibody (Cat#63184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). EIF4EBP1, eukaryotic translation initiation factor 4E binding protein 1.
Western blotting analysis using anti-EIF4EBP1 antibody (Cat#63184). EIF4EBP1 expression in wild-type (WT) and EIF4EBP1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-EIF4EBP1 antibody (Cat#63184, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using anti-EIF4EBP1 antibody (Cat#63184, 1:1,000), Top panel: wild-type (WT); Bottom panal: EIF4EBP1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; EIF4EBP1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
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