Flow cytometric analysis of BAG5 expression in C2C12 cells using anti-BAG5 antibody (Cat#3827, 1:1,000). Green, isotype control; red, BAG5.
Immunocytochemical staining of C2C12 cells with anti-BAG5 antibody (Cat#3827, 1:500) . Nuclei were stained blue with DAPI; BAG5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-BAG5 antibody (Cat#3827). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BAG5 antibody (Cat#3827, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of BAG5 expression in C2C12 cells using anti-BAG5 antibody (Cat#3827, 1:1,000). Green, isotype control; red, BAG5.
Immunocytochemical staining of C2C12 cells with anti-BAG5 antibody (Cat#3827, 1:500) . Nuclei were stained blue with DAPI; BAG5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-BAG5 antibody (Cat#3827). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BAG5 antibody (Cat#3827, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of BAG5 expression in C2C12 cells using anti-BAG5 antibody (Cat#3827, 1:1,000). Green, isotype control; red, BAG5.
Immunocytochemical staining of C2C12 cells with anti-BAG5 antibody (Cat#3827, 1:500) . Nuclei were stained blue with DAPI; BAG5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-BAG5 antibody (Cat#3827). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BAG5 antibody (Cat#3827, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
