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KO-Validated Anti-Phospho-MSK1 (S376) Rabbit Monoclonal Antibody #71200

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Flow cytometric analysis of Phospho-MSK1 (S376) expression in H9c2 cells using anti-Phospho-MSK1 (S376) antibody (Cat#71200, 1:2,000). Green, isotype control; red, Phospho-MSK1 (S376).
Immunocytochemical staining of H9c2 cells with anti-Phospho-MSK1 (S376) antibody (Cat#71200, 1:1,000). Nuclei were stained blue with DAPI; Phospho-MSK1 (S376) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-MSK1 (S376) antibody (Cat#71200). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-MSK1 (S376) antibody (Cat#71200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-phospho-MSK1 (S376) antibody (Cat#71200). Phospho-MSK1 (S376) expression in wild-type (WT) and RPS6KA5 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-MSK1 (S376) antibody (Cat#71200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    RPS6KA5
  • 货号：
    71200
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KO-Validated Anti-Phospho-MSK1 (S376) Rabbit Monoclonal Antibody
Aliases RPS6KA5; Ribosomal Protein S6 Kinase A5; MSK1; RLPK; Nuclear Mitogen- And Stress-Activated Protein Kinase 1; 90 KDa Ribosomal Protein S6 Kinase 5; Ribosomal Protein S6 Kinase Alpha-5; RSK-Like Protein Kinase; S6K-Alpha-5; EC 2.7.11.1; RSKL; Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5; Ribosomal Protein S6 Kinase, 90kD, Polypeptide 5; EC 2.7.11; MSPK1
Background

Gene Name: RPS6KA5

NCBI Gene Entry: 9252

UniProt Entry: O75582
Application Information

Molecular Weight: Predicted, 90 kDa; observed, 90 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2625

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Phospho-MSK1 (S376)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Phospho-MSK1 (S376) expression in H9c2 cells using anti-Phospho-MSK1 (S376) antibody (Cat#71200, 1:2,000). Green, isotype control; red, Phospho-MSK1 (S376).
  • Immunocytochemical staining of H9c2 cells with anti-Phospho-MSK1 (S376) antibody (Cat#71200, 1:1,000). Nuclei were stained blue with DAPI; Phospho-MSK1 (S376) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-phospho-MSK1 (S376) antibody (Cat#71200). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-MSK1 (S376) antibody (Cat#71200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-phospho-MSK1 (S376) antibody (Cat#71200). Phospho-MSK1 (S376) expression in wild-type (WT) and RPS6KA5 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-MSK1 (S376) antibody (Cat#71200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    RPS6KA5
  • 货号：
    71200
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Phospho-MSK1 (S376) expression in H9c2 cells using anti-Phospho-MSK1 (S376) antibody (Cat#71200, 1:2,000). Green, isotype control; red, Phospho-MSK1 (S376).
Immunocytochemical staining of H9c2 cells with anti-Phospho-MSK1 (S376) antibody (Cat#71200, 1:1,000). Nuclei were stained blue with DAPI; Phospho-MSK1 (S376) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-MSK1 (S376) antibody (Cat#71200). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-MSK1 (S376) antibody (Cat#71200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-phospho-MSK1 (S376) antibody (Cat#71200). Phospho-MSK1 (S376) expression in wild-type (WT) and RPS6KA5 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-MSK1 (S376) antibody (Cat#71200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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