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Anti-CD244 Mouse Monoclonal Antibody #3832

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Flow cytometric analysis of CD244 expression in C2C12 cells using anti-CD244 antibody (Cat#3832, 1:1,000). Green, isotype control; red, CD244.
Immunocytochemical staining of C2C12 cells with anti-CD244 antibody (Cat#3832, 1:500) . Nuclei were stained blue with DAPI; CD244 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar, 20 μm.
Western blotting analysis using anti-CD244 antibody (Cat#3832). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD244 antibody (Cat#3832, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    CD244
  • 货号:
    3832
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-CD244 Mouse Monoclonal Antibody
Aliases CD244; CD244 Molecule; NKR2B4; SLAMF4; NAIL; 2B4; Natural Killer Cell Receptor 2B4; Nmrk; CD244 Molecule, Natural Killer Cell Receptor 2B4; Signaling Lymphocytic Activation Molecule 4; NK Cell Type I Receptor Protein 2B4; NK Cell Activation-Inducing Ligand; SLAM Family Member 4; H2B4; NK Cell Activation Inducing Ligand NAIL; CD244 Natural Killer Cell Receptor 2B4; CD244 Antigen
Background

Gene Name: CD244

NCBI Gene Entry: 51744

UniProt Entry: Q9BZW8

Application Information

Molecular Weight: Predicted, 42 kDa; observed, 48 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB8095

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen Recombinant protein of human CD244
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:2,500
Flow Cytometry (FCM): 1:1,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of CD244 expression in C2C12 cells using anti-CD244 antibody (Cat#3832, 1:1,000). Green, isotype control; red, CD244.
  • Immunocytochemical staining of C2C12 cells with anti-CD244 antibody (Cat#3832, 1:500) . Nuclei were stained blue with DAPI; CD244 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-CD244 antibody (Cat#3832). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD244 antibody (Cat#3832, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of CD244 expression in C2C12 cells using anti-CD244 antibody (Cat#3832, 1:1,000). Green, isotype control; red, CD244.
Immunocytochemical staining of C2C12 cells with anti-CD244 antibody (Cat#3832, 1:500) . Nuclei were stained blue with DAPI; CD244 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium. Scale bar, 20 μm.
Western blotting analysis using anti-CD244 antibody (Cat#3832). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD244 antibody (Cat#3832, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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