Anti-Prolyl 4-Hydroxylase Subunit Beta Mouse Monoclonal Antibody - 产品中心

Anti-Prolyl 4-Hydroxylase Subunit Beta Mouse Monoclonal Antibody #3912

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Flow cytometric analysis of P4HB expression in HepG2 cells using anti-P4HB antibody (Cat#3912, 1:2,000). Green, isotype control; red, P4HB.

Immunocytochemical staining of HepG2 cells with anti-P4HB antibody (Cat#3912, 1:1,000). Nuclei were stained blue with DAPI; P4HB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-P4HB antibody (Cat#3912). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-P4HB antibody (Cat#3912, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

P4HB
货号：

3912
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-Prolyl 4-Hydroxylase Subunit Beta Mouse Monoclonal Antibody
Aliases	P4HB; Prolyl 4-Hydroxylase Subunit Beta; PDIA1; PDI; P4Hbeta; ERBA2L; PROHB; PO4HB; PO4DB; DSI; GIT; Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase), Beta Polypeptide; Protein Disulfide Isomerase Family A, Member 1; Protein Disulfide Isomerase-Associated 1; Cellular Thyroid Hormone-Binding Protein; Prolyl 4-Hydroxylase, Beta Polypeptide; Collagen Prolyl 4-Hydroxylase Beta; Protein Disulfide-Isomerase; EC 5.3.4.1; P55; Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase), Beta Polypeptide (Protein Disulfide Isomerase; Thyroid Hormone Binding Protein P55); Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase), Beta Polypeptide (Protein Disulfide Isomerase-Associated 1); Protein Disulfide Isomerase/Oxidoreductase; Glutathione-Insulin Transhydrogenase; Thyroid Hormone-Binding Protein P55; Testicular Secretory Protein Li 32; Protocollagen Hydroxylase; CLCRP1; PHDB
Background	Gene Name: P4HB NCBI Gene Entry: 5034 UniProt Entry: P07237
Application Information	Molecular Weight: Predicted, 57 kDa; observed, 57 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB8650 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human PDI
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of P4HB expression in HepG2 cells using anti-P4HB antibody (Cat#3912, 1:2,000). Green, isotype control; red, P4HB.
Immunocytochemical staining of HepG2 cells with anti-P4HB antibody (Cat#3912, 1:1,000). Nuclei were stained blue with DAPI; P4HB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-P4HB antibody (Cat#3912). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-P4HB antibody (Cat#3912, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

P4HB
货号：

3912
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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