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Anti-QKI Rabbit Monoclonal Antibody #3934

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Flow cytometric analysis of QKI expression in C2C12 cells using anti-QKI antibody (Cat#3934, 1:2,000). Green, isotype control; red, QKI.
Immunocytochemical staining of C2C12 cells with anti-QKI antibody (Cat#3934, 1:1,000). Nuclei were stained blue with DAPI; QKI was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-QKI antibody (Cat#3934). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-QKI antibody (Cat#3934, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human glioblastoma using anti-QKI antibody (Cat#3934, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • 基因名:
    QKI
  • 货号:
    3934
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-QKI Rabbit Monoclonal Antibody
Aliases QKI; QKI, KH Domain Containing RNA Binding; QK3; KH Domain-Containing RNA-Binding Protein QKI; HqkI; Hqk; Homolog Of Mouse Quaking QKI (KH Domain RNA Binding Protein); Quaking Homolog, KH Domain RNA Binding (Mouse); Quaking Homolog, KH Domain RNA Binding; QKI/LOC100132735 Fusion; RNA Binding Protein HQK; Protein Quaking; QK1; HKQ; QK
Background

Gene Name: QKI

NCBI Gene Entry: 9444

UniProt Entry: Q96PU8

Application Information

Molecular Weight: Predicted, 38 kDa; observed, 38 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB88760

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human QK1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of QKI expression in C2C12 cells using anti-QKI antibody (Cat#3934, 1:2,000). Green, isotype control; red, QKI.
  • Immunocytochemical staining of C2C12 cells with anti-QKI antibody (Cat#3934, 1:1,000). Nuclei were stained blue with DAPI; QKI was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-QKI antibody (Cat#3934). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-QKI antibody (Cat#3934, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human glioblastoma using anti-QKI antibody (Cat#3934, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Citation(0)
Flow cytometric analysis of QKI expression in C2C12 cells using anti-QKI antibody (Cat#3934, 1:2,000). Green, isotype control; red, QKI.
Immunocytochemical staining of C2C12 cells with anti-QKI antibody (Cat#3934, 1:1,000). Nuclei were stained blue with DAPI; QKI was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-QKI antibody (Cat#3934). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-QKI antibody (Cat#3934, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human glioblastoma using anti-QKI antibody (Cat#3934, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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