KD-Validated Anti-H2B Clustered Histone 12 Rabbit Monoclonal Antibody - 产品中心

KD-Validated Anti-H2B Clustered Histone 12 Rabbit Monoclonal Antibody #61586

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Immunocytochemical staining of HepG2 cells with anti-Histone H2B antibody (Cat#61586, 1:1,000). Nuclei were stained blue with DAPI; Histone H2B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-histone H2B antibody (Cat#61586). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-histone H2B antibody (Cat#61586, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using anti-histone H2B antibody (Cat#61586). Histone H2B expression in wild type (WT) and H2BC12 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-histone H2B antibody (Cat#61586, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

H2BC12
货号：

61586
应用：

WB, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information

Product Name	KD-Validated Anti-H2B Clustered Histone 12 Rabbit Monoclonal Antibody
Aliases	H2BC12; H2B Clustered Histone 12; HIST1H2B; H2BFAiii; H2BFT; Histone Cluster 1 H2B Family Member K; H2B Histone Family, Member T; HIRA-Interacting Protein 1; Histone Cluster 1, H2bk; Histone H2B Type 1-K; Histone 1, H2bk; H2B K; Histone Family Member; HIRIP1; H2B/S; H2BK
Background	Gene Name: H2BC12 NCBI Gene Entry: 85236 UniProt Entry: O60814
Application Information	Molecular Weight: Predicted, 14 kDa; observed, 17 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB2925 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Histone H2B
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western blotting (WB): 1:4,000-1:20,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Immunocytochemical staining of HepG2 cells with anti-Histone H2B antibody (Cat#61586, 1:1,000). Nuclei were stained blue with DAPI; Histone H2B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-histone H2B antibody (Cat#61586). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-histone H2B antibody (Cat#61586, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-histone H2B antibody (Cat#61586). Histone H2B expression in wild type (WT) and H2BC12 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-histone H2B antibody (Cat#61586, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

H2BC12
货号：

61586
应用：

WB, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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