Anti-Methylthioadenosine phosphorylase Rabbit Monoclonal Antibody - 产品中心

Anti-Methylthioadenosine phosphorylase Rabbit Monoclonal Antibody #3993

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Flow cytometric analysis of methylthioadenosine phosphorylase expression in HT-1080 cells using anti-methylthioadenosine phosphorylase antibody (Cat#3993, 1:2,000). Green, isotype control; red, methylthioadenosine phosphorylase.

Immunocytochemical staining of HT-1080 cells with anti-Methylthioadenosine phosphorylase antibody (Cat#3993, 1:1,000). Nuclei were stained blue with DAPI; Methylthioadenosine phosphorylase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-methylthioadenosine phosphorylase antibody (Cat#3993). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-methylthioadenosine phosphorylase antibody (Cat#3993, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

MTAP
货号：

3993
应用：

WB, FCM, IC
物种反应性：

M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-Methylthioadenosine phosphorylase Rabbit Monoclonal Antibody
Aliases	MTAP; Methylthioadenosine Phosphorylase; MSAP; S-Methyl-5'-Thioadenosine Phosphorylase; 5'-Methylthioadenosine Phosphorylase; MTA Phosphorylase; MTAPase; C86fus; Epididymis Secretory Sperm Binding Protein; Epididymis Luminal Protein 249; MeSAdo Phosphorylase; EC 2.4.2.28; HEL-249; DMSMFH; C86FUS; DMSFH; LGMBF; BDMF
Background	Gene Name: MTAP NCBI Gene Entry: 4507 UniProt Entry: Q13126
Application Information	Molecular Weight: Predicted, 31 kDa; observed, 29 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB9055 Species Reactivity: Mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human MTAP
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of methylthioadenosine phosphorylase expression in HT-1080 cells using anti-methylthioadenosine phosphorylase antibody (Cat#3993, 1:2,000). Green, isotype control; red, methylthioadenosine phosphorylase.
Immunocytochemical staining of HT-1080 cells with anti-Methylthioadenosine phosphorylase antibody (Cat#3993, 1:1,000). Nuclei were stained blue with DAPI; Methylthioadenosine phosphorylase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-methylthioadenosine phosphorylase antibody (Cat#3993). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-methylthioadenosine phosphorylase antibody (Cat#3993, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

MTAP
货号：

3993
应用：

WB, FCM, IC
物种反应性：

M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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