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Anti-Alkaline phosphatase Rabbit Monoclonal Antibody #4511

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Flow cytometric analysis of Alkaline phosphatase expression in HepG2 cells using anti-Alkaline phosphatase antibody (Cat#4511, 1:2,000). Green, isotype control; red, Alkaline phosphatase.

Immunocytochemical staining of HepG2 cells with anti-Alkaline phosphatase antibody (Cat#4511, 1:1,000) . Nuclei were stained blue with DAPI; Alkaline phosphatase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-alkaline phosphatase antibody (Cat#4511). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-alkaline phosphatase antibody (Cat#4511, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

ALPI
货号：

4511
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Alkaline phosphatase Rabbit Monoclonal Antibody
Aliases	ALPI; Alkaline Phosphatase, Intestinal; IAP; Intestinal Alkaline Phosphatase; Intestinal-Type Alkaline Phosphatase; EC 3.1.3.1; Alkaline Phosphomonoesterase; Glycerophosphatase; Kasahara Isozyme
Background	Gene Name: ALPI NCBI Gene Entry: 248 UniProt Entry: P09923
Application Information	Molecular Weight: Predicted, 57 kDa; observed, 68 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB11585 Species Reactivity: Human Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human Alkaline phosphatase
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Alkaline phosphatase expression in HepG2 cells using anti-Alkaline phosphatase antibody (Cat#4511, 1:2,000). Green, isotype control; red, Alkaline phosphatase.
Immunocytochemical staining of HepG2 cells with anti-Alkaline phosphatase antibody (Cat#4511, 1:1,000) . Nuclei were stained blue with DAPI; Alkaline phosphatase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-alkaline phosphatase antibody (Cat#4511). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-alkaline phosphatase antibody (Cat#4511, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

ALPI
货号：

4511
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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