Immunocytochemical staining of HT-1080 cells with anti-Glycogen synthase kinase 3 beta antibody (Cat#4019, 1:1,000). Nuclei were stained blue with DAPI; Glycogen synthase kinase 3 beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-glycogen synthase kinase 3 beta antibody (Cat#4019). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-glycogen synthase kinase 3 beta antibody (Cat#4019, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with anti-Glycogen synthase kinase 3 beta antibody (Cat#4019, 1:1,000). Nuclei were stained blue with DAPI; Glycogen synthase kinase 3 beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-glycogen synthase kinase 3 beta antibody (Cat#4019). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-glycogen synthase kinase 3 beta antibody (Cat#4019, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with anti-Glycogen synthase kinase 3 beta antibody (Cat#4019, 1:1,000). Nuclei were stained blue with DAPI; Glycogen synthase kinase 3 beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-glycogen synthase kinase 3 beta antibody (Cat#4019). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-glycogen synthase kinase 3 beta antibody (Cat#4019, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
