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Anti-Paired box 6 Rabbit Monoclonal Antibody #4247

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Flow cytometric analysis of Paired box 6 expression in HeLa cells using anti-Paired box 6 antibody (Cat#4247, 1:2,000). Green, isotype control; red, Paired box 6.

Immunocytochemical staining of HeLa cells with anti-PAX6 antibody (Cat#4247, 1:1,000). Nuclei were stained blue with DAPI; PAX6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-paired box 6 antibody (Cat#4247). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-paired box 6 antibody (Cat#4247, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

PAX6
货号：

4247
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Paired box 6 Rabbit Monoclonal Antibody
Aliases	Paired Box 6; D11S812E; WAGR; AN2; AN; Paired Box Protein Pax-6; Aniridia Type II Protein; Oculorhombin; Aniridia 1; Aniridia 2; AN1; Paired Box Gene 6 (Aniridia, Keratitis); Paired Box 6 PAX6A-ASdel6-AS7 Isoform; Paired Box 6 PAX6A-ASdel6 Isoform; Paired Box 6 PAX6B-ASdel6 Isoform; Paired Box 6 PAX6A-Del6 Isoform; Paired Box 6 PAX6-12a Isoform; Paired Box 6 PAX6D Isoform; Paired Box Homeotic Gene-6; Alternative Protein PAX6; Aniridia, Keratitis; ASGD5; FVH1; MGDA;
Background	Gene Name: PAX6 NCBI Gene Entry: 5080 UniProt Entry: P26367
Application Information	Molecular Weight: Predicted, 55 kDa; observed, 47 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB10265 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PAX6
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Paired box 6 expression in HeLa cells using anti-Paired box 6 antibody (Cat#4247, 1:2,000). Green, isotype control; red, Paired box 6.
Immunocytochemical staining of HeLa cells with anti-PAX6 antibody (Cat#4247, 1:1,000). Nuclei were stained blue with DAPI; PAX6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-paired box 6 antibody (Cat#4247). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-paired box 6 antibody (Cat#4247, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

PAX6
货号：

4247
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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