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Anti-NF-kB p65 Rabbit Monoclonal Antibody #4029

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Flow cytometric analysis of NF-kB p65 expression in HepG2 cells using anti-NF-kB p65 antibody (Cat#4029, 1:2,000). Green, isotype control; red, NF-kB p65.

Immunocytochemical staining of HepG2 cells with anti-NF-kB p65 antibody (Cat#4029, 1:1,000). Nuclei were stained blue with DAPI; NF-kB p65 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-NF-kB p65 antibody (Cat#4029). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NF-kB p65 antibody (Cat#4029, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

RELA
货号：

4029
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Citation(0)

Information

Product Name	Anti-NF-kB p65 Rabbit Monoclonal Antibody
Aliases	RELA; RELA Proto-Oncogene, NF-KB Subunit; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells; NFKB3; P65; V-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A; Nuclear Factor NF-Kappa-B P65 Subunit; Transcription Factor P65; NF-Kappa-B Transcription Factor P65; NF-Kappa-B; P65delta3; AIF3BL3; CMCU
Background	Gene Name: RELA NCBI Gene Entry: 5970 UniProt Entry: Q04206
Application Information	Molecular Weight: Predicted, 60 kDa; observed, 65 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB9235 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human NF-κB p65
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:2,000-1:10,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of NF-kB p65 expression in HepG2 cells using anti-NF-kB p65 antibody (Cat#4029, 1:2,000). Green, isotype control; red, NF-kB p65.
Immunocytochemical staining of HepG2 cells with anti-NF-kB p65 antibody (Cat#4029, 1:1,000). Nuclei were stained blue with DAPI; NF-kB p65 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-NF-kB p65 antibody (Cat#4029). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NF-kB p65 antibody (Cat#4029, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

RELA
货号：

4029
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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