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Anti-GPR97 Rabbit Polyclonal Antibody #51649

一键复制产品信息
Immunocytochemical analysis of GPR97 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of GPR97 expression in HepG2 (A), SP20 (B) whole cell lysates.
  • 基因名:
    GPR97
  • 货号:
    51649
  • 应用:
    WB, IC
  • 物种反应性:
    H, M
规格:
20 μL,50 μL,100 μL
价格:
¥ 560.00,¥ 1,100.00,¥ 2,000.00
Information
Product Name Anti-GPR97 Rabbit Polyclonal Antibody
Aliases PGR26; Probable G-protein coupled receptor 97; G-protein coupled receptor PGR26
Background

Gene Name: GPR97

NCBI Gene Entry: 222487

UniProt Entry: Q86Y34

Application Information

Molecular Weight: Predicted, 60 kDa; observed, 60,104 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human GPR97
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunocytochemistry (IC): 1:100-1:500
Note This product is for research use only.
Picture
  • Immunocytochemical analysis of GPR97 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
  • Western blotting analysis of GPR97 expression in HepG2 (A), SP20 (B) whole cell lysates.
Citation(0)
Immunocytochemical analysis of GPR97 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of GPR97 expression in HepG2 (A), SP20 (B) whole cell lysates.
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