Flow cytometric analysis of Flotillin1 expression in HepG2 cells using anti-Flotillin1 antibody (Cat#4080, 1:2,000). Green, isotype control; red, Flotillin1.
Immunocytochemical staining of HepG2 cells with anti-Flotillin 1 antibody (Cat#4080, 1:1,000) . Nuclei were stained blue with DAPI; Flotillin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-flotillin 1 antibody (Cat#4080). Total cell lysates (30 μg for H9c2, 20 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-flotillin 1 antibody (Cat#4080, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Flotillin1 expression in HepG2 cells using anti-Flotillin1 antibody (Cat#4080, 1:2,000). Green, isotype control; red, Flotillin1.
Immunocytochemical staining of HepG2 cells with anti-Flotillin 1 antibody (Cat#4080, 1:1,000) . Nuclei were stained blue with DAPI; Flotillin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-flotillin 1 antibody (Cat#4080). Total cell lysates (30 μg for H9c2, 20 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-flotillin 1 antibody (Cat#4080, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Flotillin1 expression in HepG2 cells using anti-Flotillin1 antibody (Cat#4080, 1:2,000). Green, isotype control; red, Flotillin1.
Immunocytochemical staining of HepG2 cells with anti-Flotillin 1 antibody (Cat#4080, 1:1,000) . Nuclei were stained blue with DAPI; Flotillin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-flotillin 1 antibody (Cat#4080). Total cell lysates (30 μg for H9c2, 20 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-flotillin 1 antibody (Cat#4080, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
