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Anti-ATG5 Rabbit Monoclonal Antibody #4205

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Flow cytometric analysis of ATG5 expression in HepG2 cells using anti-ATG5 antibody (Cat#4205, 1:2,000). Green, isotype control; red, ATG5.

Immunocytochemical staining of HepG2 cells with anti-ATG5 antibody (Cat#4205, 1:1,000). Nuclei were stained blue with DAPI; ATG5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-ATG5 antibody (Cat#4205). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATG5 antibody (Cat#4205, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

ATG5
货号：

4205
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-ATG5 Rabbit Monoclonal Antibody
Aliases	ATG5; Autophagy Related 5; ASP; HAPG5; APG5L; APG5; Apoptosis-Specific Protein; Autophagy Protein 5; ATG5 Autophagy Related 5 Homolog (S. Cerevisiae); APG5 (Autophagy 5, S. Cerevisiae)-Like; APG5 Autophagy 5-Like (S. Cerevisiae); ATG5 Autophagy Related 5 Homolog; APG5 Autophagy 5-Like; APG5-LIKE; APG5-Like; SCAR25
Background	Gene Name: ATG5 NCBI Gene Entry: 9474 UniProt Entry: Q9H1Y0
Application Information	Molecular Weight: Predicted, 32 kDa; observed, 50 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB10060 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human ATG5
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of ATG5 expression in HepG2 cells using anti-ATG5 antibody (Cat#4205, 1:2,000). Green, isotype control; red, ATG5.
Immunocytochemical staining of HepG2 cells with anti-ATG5 antibody (Cat#4205, 1:1,000). Nuclei were stained blue with DAPI; ATG5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-ATG5 antibody (Cat#4205). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATG5 antibody (Cat#4205, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

ATG5
货号：

4205
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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