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Anti-Histone deacetylase 9 Rabbit Monoclonal Antibody #4238

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Flow cytometric analysis of histone deacetylase 9 expression in HepG2 cells using anti-histone deacetylase 9 antibody (Cat#4238, 1:2,000). Green, isotype control; red, histone deacetylase 9.
Immunocytochemical staining of HepG2 cells with anti-Histone deacetylase 9 antibody (Cat#4238, 1:1,000). Nuclei were stained blue with DAPI; Histone deacetylase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-histone deacetylase 9 antibody (Cat#4238). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-histone deacetylase 9 antibody (Cat#4238, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    HDAC9
  • 货号:
    4238
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Histone deacetylase 9 Rabbit Monoclonal Antibody
Aliases HDAC9; Histone Deacetylase 9; HDAC7B; MITR; HD7; KIAA0744; HDAC; Histone Deacetylase 7B; EC 3.5.1.98; HDAC; HD7b; HDRP; HD9; MEF-2 Interacting Transcription Repressor (MITR) Protein; MEF2-Interacting Transcription Repressor MITR; Histone Deacetylase 4/5-Related Protein; Histone Deacetylase-Related Protein; HDAC9FL; ARCND4; HDAC9B
Background

Gene Name: HDAC9

NCBI Gene Entry: 9734

UniProt Entry: Q9UKV0

Application Information

Molecular Weight: Predicted, 111 kDa; observed, 160 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10225

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human HDAC9
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:4,000-1:20,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of histone deacetylase 9 expression in HepG2 cells using anti-histone deacetylase 9 antibody (Cat#4238, 1:2,000). Green, isotype control; red, histone deacetylase 9.
  • Immunocytochemical staining of HepG2 cells with anti-Histone deacetylase 9 antibody (Cat#4238, 1:1,000). Nuclei were stained blue with DAPI; Histone deacetylase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-histone deacetylase 9 antibody (Cat#4238). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-histone deacetylase 9 antibody (Cat#4238, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of histone deacetylase 9 expression in HepG2 cells using anti-histone deacetylase 9 antibody (Cat#4238, 1:2,000). Green, isotype control; red, histone deacetylase 9.
Immunocytochemical staining of HepG2 cells with anti-Histone deacetylase 9 antibody (Cat#4238, 1:1,000). Nuclei were stained blue with DAPI; Histone deacetylase 9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-histone deacetylase 9 antibody (Cat#4238). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-histone deacetylase 9 antibody (Cat#4238, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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