Flow cytometric analysis of NFKB inhibitor alpha expression in HepG2 cells using NFKB inhibitor alpha antibody (Cat#4241, 1:2,000). Green, isotype control; red, NFKB inhibitor alpha.
Immunocytochemical staining of HepG2 cells with NFKB inhibitor alpha antibody (4241, 1:1,000). Nuclei were stained blue with DAPI; NFKB inhibitor alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-NFKB inhibitor alpha antibody (Cat#4241). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NFKB inhibitor alpha antibody (Cat#4241, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of NFKB inhibitor alpha expression in HepG2 cells using NFKB inhibitor alpha antibody (Cat#4241, 1:2,000). Green, isotype control; red, NFKB inhibitor alpha.
Immunocytochemical staining of HepG2 cells with NFKB inhibitor alpha antibody (4241, 1:1,000). Nuclei were stained blue with DAPI; NFKB inhibitor alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-NFKB inhibitor alpha antibody (Cat#4241). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NFKB inhibitor alpha antibody (Cat#4241, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of NFKB inhibitor alpha expression in HepG2 cells using NFKB inhibitor alpha antibody (Cat#4241, 1:2,000). Green, isotype control; red, NFKB inhibitor alpha.
Immunocytochemical staining of HepG2 cells with NFKB inhibitor alpha antibody (4241, 1:1,000). Nuclei were stained blue with DAPI; NFKB inhibitor alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-NFKB inhibitor alpha antibody (Cat#4241). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NFKB inhibitor alpha antibody (Cat#4241, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
