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Anti-ATP6V0D1 Rabbit Monoclonal Antibody #3676

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Flow cytometric analysis of ATP6V0D1 expression in HepG2 cells using anti-ATP6V0D1 antibody (Cat#3676, 1:2,000). Green, isotype control; red, ATP6V0D1.

Immunocytochemical staining of HepG2 cells with anti-ATP6V0D1 antibody (Cat#3676, 1:1,000) . Nuclei were stained blue with DAPI; ATP6V0D1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-ATP6V0D1 antibody (Cat#3676). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP6V0D1 antibody (Cat#3676, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

ATP6V0D1
货号：

3676
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Citation(0)

Information

Product Name	Anti-ATP6V0D1 Rabbit Monoclonal Antibody
Aliases	ATP6V0D1; ATPase H+ Transporting V0 Subunit D1; VPATPD; P39; ATP6DV; ATP6D; VATX; Vma6; ATPase, H+ Transporting, Lysosomal (Vacuolar Proton Pump), Member D; ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D1; V-ATPase 40 KDa Accessory Protein; V-Type Proton ATPase Subunit D 1; Vacuolar Proton Pump Subunit D 1; 32 KDa Accessory Protein; V-ATPase AC39 Subunit; V-ATPase Subunit D 1; ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D Isoform 1; H(+)-Transporting Two-Sector ATPase, Subunit D; V-ATPase Subunit D1; V-ATPase, Subunit D
Background	Gene Name: ATP6V0D1 NCBI Gene Entry: 9114 UniProt Entry: P61421
Application Information	Molecular Weight: Predicted, 40 kDa; observed, 37 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB6855 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human ATP6V0D1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of ATP6V0D1 expression in HepG2 cells using anti-ATP6V0D1 antibody (Cat#3676, 1:2,000). Green, isotype control; red, ATP6V0D1.
Immunocytochemical staining of HepG2 cells with anti-ATP6V0D1 antibody (Cat#3676, 1:1,000) . Nuclei were stained blue with DAPI; ATP6V0D1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-ATP6V0D1 antibody (Cat#3676). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP6V0D1 antibody (Cat#3676, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

ATP6V0D1
货号：

3676
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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