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Anti-CDC25C Rabbit Monoclonal Antibody #4252

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Flow cytometric analysis of CDC25C expression in HeLa cells using anti-CDC25C antibody (Cat#4252, 1:2,000). Green, isotype control; red, CDC25C.

Immunocytochemical staining of Hela cells with anti-CDC25C antibody (Cat#4252, 1:1,000) . Nuclei were stained blue with DAPI; CDC25C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-CDC25C antibody (Cat#4252). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDC25C antibody (Cat#4252, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CDC25C
货号：

4252
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-CDC25C Rabbit Monoclonal Antibody
Aliases	CDC25C; Cell Division Cycle 25C; M-Phase Inducer Phosphatase 3; PPP1R60; Protein Phosphatase 1, Regulatory Subunit 60; EC 3.1.3.48; CDC25; Dual Specificity Phosphatase CDC25C; Dual Specificity Phosphatase Cdc25C; Phosphotyrosine Phosphatase; CDC25 Homolog C (S. Pombe); Mitosis Inducer CDC25; CDC25 Homolog C
Background	Gene Name: CDC25C NCBI Gene Entry: 995 UniProt Entry: P30307
Application Information	Molecular Weight: Predicted, 53 kDa; observed, 60 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB10290 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Cdc25C
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of CDC25C expression in HeLa cells using anti-CDC25C antibody (Cat#4252, 1:2,000). Green, isotype control; red, CDC25C.
Immunocytochemical staining of Hela cells with anti-CDC25C antibody (Cat#4252, 1:1,000) . Nuclei were stained blue with DAPI; CDC25C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-CDC25C antibody (Cat#4252). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDC25C antibody (Cat#4252, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

CDC25C
货号：

4252
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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