Flow cytometric analysis of CDC40 expression in HT-1080 cells using anti-CDC40 antibody (Cat#4257, 1:2,000). Green, isotype control; red, CDC40.
Immunocytochemical staining of HT-1080 cells with anti-CDC40 antibody (Cat#4257, 1:1,000) . Nuclei were stained blue with DAPI; CDC40 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Western blotting analysis using anti-CDC40 antibody (Cat#4257). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDC40 antibody (Cat#4257, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CDC40 expression in HT-1080 cells using anti-CDC40 antibody (Cat#4257, 1:2,000). Green, isotype control; red, CDC40.
Immunocytochemical staining of HT-1080 cells with anti-CDC40 antibody (Cat#4257, 1:1,000) . Nuclei were stained blue with DAPI; CDC40 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Western blotting analysis using anti-CDC40 antibody (Cat#4257). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDC40 antibody (Cat#4257, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CDC40 expression in HT-1080 cells using anti-CDC40 antibody (Cat#4257, 1:2,000). Green, isotype control; red, CDC40.
Immunocytochemical staining of HT-1080 cells with anti-CDC40 antibody (Cat#4257, 1:1,000) . Nuclei were stained blue with DAPI; CDC40 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Western blotting analysis using anti-CDC40 antibody (Cat#4257). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDC40 antibody (Cat#4257, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
