Flow cytometric analysis of NDRG1 expression in HT-1080 cells using anti-NDRG1 antibody (Cat#63899, 1：2000). Green, isotype control; red, NDRG1.
Immunocytochemical staining of HepG2 cells with anti-NDRG1 antibody (Cat#63899, 1:1,000) . Nuclei were stained blue with DAPI; NDRG1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-NDRG1 antibody (Cat#63899). Total cell lysates (30 μ g) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDRG1 antibody (Cat#63899, 1:5,000) and HRP-conjugated goat anti- rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-NDRG1 antibody (Cat#63899). NDRG1 expression in wild type (WT) and NDRG1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NDRG1 antibody (Cat#63899, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ ™ ECL Substrate Kit (Cat#716).
