Flow cytometric analysis of phospho-STAT3 (Tyr705) expression in H9c2 cells using anti-phospho-STAT3 (Tyr705) antibody (Cat#4268, 1:2,000). Green, isotype control; red, phospho-STAT3 (Tyr705).
Immunocytochemical staining of H9C2 cells with anti-phospho-STAT3 (Tyr705) antibody (Cat#4268, 1:1,000). Nuclei were stained blue with DAPI; Phospho-STAT3 (Tyr705) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-STAT3 (Tyr705) antibody (Cat#4268). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-STAT3 (Tyr705) antibody (Cat#4268, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
STAT3; Signal Transducer And Activator Of Transcription 3; APRF; Acute-Phase Response Factor; Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor); DNA-Binding Protein APRF; ADMIO1; ADMIO; HIES
Flow cytometric analysis of phospho-STAT3 (Tyr705) expression in H9c2 cells using anti-phospho-STAT3 (Tyr705) antibody (Cat#4268, 1:2,000). Green, isotype control; red, phospho-STAT3 (Tyr705).
Immunocytochemical staining of H9C2 cells with anti-phospho-STAT3 (Tyr705) antibody (Cat#4268, 1:1,000). Nuclei were stained blue with DAPI; Phospho-STAT3 (Tyr705) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-STAT3 (Tyr705) antibody (Cat#4268). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-STAT3 (Tyr705) antibody (Cat#4268, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of phospho-STAT3 (Tyr705) expression in H9c2 cells using anti-phospho-STAT3 (Tyr705) antibody (Cat#4268, 1:2,000). Green, isotype control; red, phospho-STAT3 (Tyr705).
Immunocytochemical staining of H9C2 cells with anti-phospho-STAT3 (Tyr705) antibody (Cat#4268, 1:1,000). Nuclei were stained blue with DAPI; Phospho-STAT3 (Tyr705) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-STAT3 (Tyr705) antibody (Cat#4268). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-STAT3 (Tyr705) antibody (Cat#4268, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
