Flow cytometric analysis of E2F transcription factor 1 expression in C2C12 cells using anti-E2F transcription factor 1 antibody (Cat#4273, 1:2,000). Green, isotype control; red, E2F transcription factor 1.
Immunocytochemical staining of C2C12 cells with anti-E2F transcription factor 1 antibody (Cat#4273, 1:1,000) . Nuclei were stained blue with DAPI; E2F transcription factor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-E2F transcription factor 1 antibody (Cat#4273). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-E2F transcription factor 1 antibody (Cat#4273, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of E2F transcription factor 1 expression in C2C12 cells using anti-E2F transcription factor 1 antibody (Cat#4273, 1:2,000). Green, isotype control; red, E2F transcription factor 1.
Immunocytochemical staining of C2C12 cells with anti-E2F transcription factor 1 antibody (Cat#4273, 1:1,000) . Nuclei were stained blue with DAPI; E2F transcription factor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-E2F transcription factor 1 antibody (Cat#4273). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-E2F transcription factor 1 antibody (Cat#4273, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of E2F transcription factor 1 expression in C2C12 cells using anti-E2F transcription factor 1 antibody (Cat#4273, 1:2,000). Green, isotype control; red, E2F transcription factor 1.
Immunocytochemical staining of C2C12 cells with anti-E2F transcription factor 1 antibody (Cat#4273, 1:1,000) . Nuclei were stained blue with DAPI; E2F transcription factor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-E2F transcription factor 1 antibody (Cat#4273). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-E2F transcription factor 1 antibody (Cat#4273, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
