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Anti-Drosha ribonuclease III Rabbit Monoclonal Antibody #4324

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Flow cytometric analysis of drosha ribonuclease III expression in HepG2 cells using anti-drosha ribonuclease III antibody (Cat#4324, 1:2,000). Green, isotype control; red, drosha ribonuclease III.
Immunocytochemical staining of HepG2 cells with anti-drosha ribonuclease III antibody (Cat#4324, 1:1,000). Nuclei were stained blue with DAPI; Drosha ribonuclease III was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-drosha ribonuclease III antibody (Cat#4324). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-drosha ribonuclease III antibody (Cat#4324, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    DROSHA
  • 货号:
    4324
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Drosha ribonuclease III Rabbit Monoclonal Antibody
Aliases DROSHA; Drosha Ribonuclease III; RNASE3L; RN3; HSA242976; RNASEN; Drosha, Double-Stranded RNA-Specific Endoribonuclease; Ribonuclease Type III, Nuclear; Ribonuclease 3; EC 3.1.26.3; RNase III; ETOHI2; P241; Putative Protein P241 Which Interacts With Transcription Factor Sp1; Drosha, Ribonuclease Type III; Putative Ribonuclease III; Nuclear RNase III Drosha; Ribonuclease III; Protein Drosha; RANSE3L; Etohi2
Background

Gene Name: DROSHA

NCBI Gene Entry: 29102

UniProt Entry: Q9NRR4

Application Information

Molecular Weight: Predicted, 159 kDa; observed, 159 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10650

Species Reactivity: Human, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human Drosha
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of drosha ribonuclease III expression in HepG2 cells using anti-drosha ribonuclease III antibody (Cat#4324, 1:2,000). Green, isotype control; red, drosha ribonuclease III.
  • Immunocytochemical staining of HepG2 cells with anti-drosha ribonuclease III antibody (Cat#4324, 1:1,000). Nuclei were stained blue with DAPI; Drosha ribonuclease III was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-drosha ribonuclease III antibody (Cat#4324). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-drosha ribonuclease III antibody (Cat#4324, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of drosha ribonuclease III expression in HepG2 cells using anti-drosha ribonuclease III antibody (Cat#4324, 1:2,000). Green, isotype control; red, drosha ribonuclease III.
Immunocytochemical staining of HepG2 cells with anti-drosha ribonuclease III antibody (Cat#4324, 1:1,000). Nuclei were stained blue with DAPI; Drosha ribonuclease III was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-drosha ribonuclease III antibody (Cat#4324). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-drosha ribonuclease III antibody (Cat#4324, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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