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KD-Validated Anti-Arg2 Rabbit Monoclonal Antibody #69632

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Flow cytometric analysis of Arg2 expression in HT-1080 cells using Arg2 antibody (Cat#69632, 1:2,000). Green, isotype control; red, Arg2.

Immunocytochemical staining of HT-1080 cells with Arg2 antibody (Cat#69632, 1:1,000). Nuclei were stained blue with DAPI; Arg2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Arg2 antibody (Cat#69632). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Arg2 antibody (Cat#69632, 1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using anti-Arg2 antibody (Cat#69632). Arg2 expression in wild type (WT) and Arg2 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with anti-Arg2 antibody (Cat#69632, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

ARG2
货号：

69632
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

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Information

Product Name	KD-Validated Anti-Arg2 Rabbit Monoclonal Antibody
Aliases	ARG2; Arginase 2; Arginase-2, Mitochondrial; Kidney-Type Arginase; Non-Hepatic Arginase; Arginase, Type II; Type II Arginase; Arginase II; EC 3.5.3.1; L-Arginine Amidinohydrolase; L-Arginine Ureahydrolase; Nonhepatic Arginase; Kidney Arginase; EC 3.5.3
Background	Gene Name: ARG2 NCBI Gene Entry: 384 UniProt Entry: P78540
Application Information	Molecular Weight: Predicted, 39 kDa, observed, 39 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB655 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Arg2
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:2,000-1:10,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Arg2 expression in HT-1080 cells using Arg2 antibody (Cat#69632, 1:2,000). Green, isotype control; red, Arg2.
Immunocytochemical staining of HT-1080 cells with Arg2 antibody (Cat#69632, 1:1,000). Nuclei were stained blue with DAPI; Arg2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Arg2 antibody (Cat#69632). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Arg2 antibody (Cat#69632, 1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Arg2 antibody (Cat#69632). Arg2 expression in wild type (WT) and Arg2 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with anti-Arg2 antibody (Cat#69632, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

ARG2
货号：

69632
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 650.00，¥ 1,350.00，¥ 2,500.00

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