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Anti-CD276 Rabbit Monoclonal Antibody #4329

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Flow cytometric analysis of CD276 expression in Hela cells using anti-CD276 antibody (Cat#4329, 1:2,000). Green, isotype control; red, CD276.
Immunocytochemical staining of Hela cells with anti-CD276 antibody (Cat#4329, 1:1,000). Nuclei were stained blue with DAPI; CD276 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-CD276 antibody (Cat#4329). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD276 antibody (Cat#4329, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    CD276
  • 货号：
    4329
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-CD276 Rabbit Monoclonal Antibody
Aliases CD276; CD276 Molecule; B7-H3; B7H3; CD276 Antigen; B7RP-2; Costimulatory Molecule; B7 Homolog 3; 4Ig-B7-H3
Background

Gene Name: CD276

NCBI Gene Entry: 80381

UniProt Entry: Q5ZPR3
Application Information

Molecular Weight: Predicted, 57 kDa; observed, 100 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10675

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human CD276
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of CD276 expression in Hela cells using anti-CD276 antibody (Cat#4329, 1:2,000). Green, isotype control; red, CD276.
  • Immunocytochemical staining of Hela cells with anti-CD276 antibody (Cat#4329, 1:1,000). Nuclei were stained blue with DAPI; CD276 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-CD276 antibody (Cat#4329). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD276 antibody (Cat#4329, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    CD276
  • 货号：
    4329
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of CD276 expression in Hela cells using anti-CD276 antibody (Cat#4329, 1:2,000). Green, isotype control; red, CD276.
Immunocytochemical staining of Hela cells with anti-CD276 antibody (Cat#4329, 1:1,000). Nuclei were stained blue with DAPI; CD276 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-CD276 antibody (Cat#4329). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD276 antibody (Cat#4329, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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