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Anti-APE1 Rabbit Monoclonal Antibody #4347

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Flow cytometric analysis of APE1 expression in HT-1080 cells using anti-APE1 antibody (Cat#4347, 1:2,000). Green, isotype control; red, APE1.
Western blotting analysis using anti-APE1 antibody (Cat#4347). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-APE1 antibody (Cat#4347, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    APEX1
  • 货号:
    4347
  • 应用:
    WB, FCM
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-APE1 Rabbit Monoclonal Antibody
Aliases APEX1; Apurinic/Apyrimidinic Endodeoxyribonuclease 1; REF1; HAP1; APEN; APE; APX; REF-1; APE-1; APEX; APEX Nuclease (Multifunctional DNA Repair Enzyme) 1; DNA-(Apurinic Or Apyrimidinic Site) Endonuclease; DNA Repair Nuclease/Redox Regulator APEX1; Apurinic-Apyrimidinic Endonuclease 1; Redox Factor-1; APE1; APEX Nuclease (Multifunctional DNA Repair Enzyme); Deoxyribonuclease (Apurinic Or Apyrimidinic); Apurinic/Apyrimidinic (Abasic) Endonuclease; DNA-(Apurinic Or Apyrimidinic Site) Lyase; AP Endonuclease Class I; AP Endonuclease 1; Protein REF-1; APEX Nuclease; EC 4.2.99.18; EC 3.1.11.2; EC 3.1.21.-; AP Lyase
Background

Gene Name: APEX1

NCBI Gene Entry: 328

UniProt Entry: P27695

Application Information

Molecular Weight: Predicted, 36 kDa; observed, 36 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10765

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry(FCM)

Immunogen A synthesized peptide derived from human APEX1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:200 - 1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of APE1 expression in HT-1080 cells using anti-APE1 antibody (Cat#4347, 1:2,000). Green, isotype control; red, APE1.
  • Western blotting analysis using anti-APE1 antibody (Cat#4347). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-APE1 antibody (Cat#4347, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of APE1 expression in HT-1080 cells using anti-APE1 antibody (Cat#4347, 1:2,000). Green, isotype control; red, APE1.
Western blotting analysis using anti-APE1 antibody (Cat#4347). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-APE1 antibody (Cat#4347, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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