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Anti-Calpain 2 Rabbit Monoclonal Antibody #4356

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Flow cytometric analysis of calpain 2 expression in HT-1080 cells using anti-calpain 2 antibody (Cat#4356, 1:2,000). Green, isotype control; red, calpain 2.

Immunocytochemical staining of HT-1080 cells with anti-Calpain 2 antibody (Cat#4356, 1:1,000) . Nuclei were stained blue with DAPI; Calpain 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-calpain 2 antibody (Cat#4356). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-calpain 2 antibody (Cat#4356, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

CAPN2
货号：

4356
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Calpain 2 Rabbit Monoclonal Antibody
Aliases	CAPN2; Calpain 2; CANPL2; MCANP; Calcium-Activated Neutral Proteinase; Calpain 2, (M/II) Large Subunit; Calpain-2 Catalytic Subunit; Millimolar-Calpain; Calpain M-Type; EC 3.4.22.53; M-Calpain; CANPml; CANP; Calpain 2, Large [Catalytic] Subunit; Calpain, Large Polypeptide L2; Calpain Large Polypeptide L2; Calpain 2, Large Subunit; Calpain-2 Large Subunit; EC 3.4.22; CANPML; CANP2
Background	Gene Name: CAPN2 NCBI Gene Entry: 824 UniProt Entry: P17655
Application Information	Molecular Weight: Predicted, 80 kDa; observed, 73 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB10810 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Calpain 2
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of calpain 2 expression in HT-1080 cells using anti-calpain 2 antibody (Cat#4356, 1:2,000). Green, isotype control; red, calpain 2.
Immunocytochemical staining of HT-1080 cells with anti-Calpain 2 antibody (Cat#4356, 1:1,000) . Nuclei were stained blue with DAPI; Calpain 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-calpain 2 antibody (Cat#4356). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-calpain 2 antibody (Cat#4356, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

CAPN2
货号：

4356
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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