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Anti-MAP3K7 Rabbit Monoclonal Antibody #4370

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Flow cytometric analysis of MAP3K7 expression in HeLa cells using anti-MAP3K7 antibody (Cat#4370, 1:2,000). Green, isotype control; red, MAP3K7.
Immunocytochemical staining of Hela cells with anti-MAP3K7 antibody (Cat#4370, 1:1,000). Nuclei were stained blue with DAPI; MAP3K7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar, 20 μm.
Western blotting analysis using anti-MAP3K7 antibody (Cat#4370). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAP3K7 antibody (Cat#4370, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    MAP3K7
  • 货号:
    4370
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-MAP3K7 Rabbit Monoclonal Antibody
Aliases MAP3K7; Mitogen-Activated Protein Kinase Kinase Kinase 7; MEKK7; TAK1; Transforming Growth Factor-Beta-Activated Kinase 1; TGF-Beta Activated Kinase 1; EC 2.7.11.25; TGF-Beta-Activated Kinase 1; EC 2.7.11; TGF1a; CSCF; FMD2
Background

Gene Name: MAP3K7

NCBI Gene Entry: 6885

UniProt Entry: O43318

Application Information

Molecular Weight: Predicted, 67 kDa; observed, 75 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10880

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human TAK1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of MAP3K7 expression in HeLa cells using anti-MAP3K7 antibody (Cat#4370, 1:2,000). Green, isotype control; red, MAP3K7.
  • Immunocytochemical staining of Hela cells with anti-MAP3K7 antibody (Cat#4370, 1:1,000). Nuclei were stained blue with DAPI; MAP3K7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar, 20 μm.
  • Western blotting analysis using anti-MAP3K7 antibody (Cat#4370). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAP3K7 antibody (Cat#4370, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of MAP3K7 expression in HeLa cells using anti-MAP3K7 antibody (Cat#4370, 1:2,000). Green, isotype control; red, MAP3K7.
Immunocytochemical staining of Hela cells with anti-MAP3K7 antibody (Cat#4370, 1:1,000). Nuclei were stained blue with DAPI; MAP3K7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar, 20 μm.
Western blotting analysis using anti-MAP3K7 antibody (Cat#4370). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MAP3K7 antibody (Cat#4370, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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