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Anti-Phospho-FOXO1/3/4 (T24/32) Rabbit Polyclonal Antibody #U0881

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Western blotting analysis using anti-Phospho-FOXO1/3/4 (T24/32) antibody (Cat#U0881). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-FOXO1/3/4 (T24/32) antibody (Cat#U0881, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemical analysis of FOXO1/3/4 (Phospho-T24/32) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • 基因名：
    FOXO1/3/4
  • 货号：
    U0881
  • 应用：
    WB, IHC
  • 物种反应性：
    H, M, R, B, P, Z
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00

Information

Picture

Citation(0)

Information
Product Name Anti-Phospho-FOXO1/3/4 (T24/32) Rabbit Polyclonal Antibody
Aliases FOXO1; FKHR; FOXO1A; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; FOXO3; FKHRL1; FOXO3A; Forkhead box protein O3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO4; AFX; AFX1; MLLT7; Forkhead box protein O4; Fork head domain transcription factor AFX1
Background

Gene Name: FOXO1/3/4

NCBI Gene Entry: 2308/4303

UniProt Entry: Q12778/O43524/P98177
Application Information

Molecular Weight: Predicted, 69, 71, 53 kDa; observed, 95 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, bovine, pig, zebrafish

Applications Tested: Western blotting (WB), immunohistochemistry (IHC)
Immunogen A synthesized peptide derived from human Phospho-FOXO1/3/4 (T24/32)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunohistochemistry (IHC): 1:50-1:200
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-Phospho-FOXO1/3/4 (T24/32) antibody (Cat#U0881). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-FOXO1/3/4 (T24/32) antibody (Cat#U0881, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemical analysis of FOXO1/3/4 (Phospho-T24/32) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Citation(0)
  • 基因名：
    FOXO1/3/4
  • 货号：
    U0881
  • 应用：
    WB, IHC
  • 物种反应性：
    H, M, R, B, P, Z
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00
RELATED PRODUCTS
Western blotting analysis using anti-Phospho-FOXO1/3/4 (T24/32) antibody (Cat#U0881). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-FOXO1/3/4 (T24/32) antibody (Cat#U0881, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemical analysis of FOXO1/3/4 (Phospho-T24/32) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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