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Anti-CD82 Rabbit Monoclonal Antibody #4373

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Flow cytometric analysis of CD82 expression in HT-1080 cells using anti-CD82 antibody (Cat#4373, 1:2,000). Green, isotype control; red, CD82.
Immunocytochemical staining of HT-1080 cells with anti-CD82 antibody (Cat#4373, 1:1,000) . Nuclei were stained blue with DAPI; CD82 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-CD82 antibody (Cat#4373). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD82 antibody (Cat#4373, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    CD82
  • 货号：
    4373
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-CD82 Rabbit Monoclonal Antibody
Aliases CD82; CD82 Molecule; TSPAN27; IA4; CD82 Antigen; KAI1; ST6; R2; Kangai 1 (Suppression Of Tumorigenicity 6, Prostate; CD82 Antigen (R2 Leukocyte Antigen, Antigen Detected By Monoclonal And Antibody IA4)); Metastasis Suppressor Kangai-1; Inducible Membrane Protein R2; Tetraspanin-27; C33 Antigen; Tspan-27; SAR2; Suppressor Of Tumorigenicity 6 Protein; Suppression Of Tumorigenicity 6; R2 Leukocyte Antigen; GR15; 4F9; C33
Background

Gene Name: CD82

NCBI Gene Entry: 3732

UniProt Entry: P27701
Application Information

Molecular Weight: Predicted, 30 kDa; observed, 30-60 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10895

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human CD82
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of CD82 expression in HT-1080 cells using anti-CD82 antibody (Cat#4373, 1:2,000). Green, isotype control; red, CD82.
  • Immunocytochemical staining of HT-1080 cells with anti-CD82 antibody (Cat#4373, 1:1,000) . Nuclei were stained blue with DAPI; CD82 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-CD82 antibody (Cat#4373). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD82 antibody (Cat#4373, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    CD82
  • 货号：
    4373
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of CD82 expression in HT-1080 cells using anti-CD82 antibody (Cat#4373, 1:2,000). Green, isotype control; red, CD82.
Immunocytochemical staining of HT-1080 cells with anti-CD82 antibody (Cat#4373, 1:1,000) . Nuclei were stained blue with DAPI; CD82 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-CD82 antibody (Cat#4373). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD82 antibody (Cat#4373, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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