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Anti-DNA ligase 4 Rabbit Monoclonal Antibody #4394

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Flow cytometric analysis of DNA ligase 4 expression in HeLa cells using anti-DNA ligase 4 antibody (Cat#4394, 1:2,000). Green, isotype control; red, DNA ligase 4.

Immunocytochemical staining of Hela cells with anti-DNA ligase 4 antibody (Cat#4394, 1:1,000). Nuclei were stained blue with DAPI; DNA ligase 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using anti-DNA ligase 4 antibody (Cat#4394). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNA ligase 4 antibody (Cat#4394, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

LIG4
货号：

4394
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-DNA ligase 4 Rabbit Monoclonal Antibody
Aliases	LIG4; DNA Ligase 4; Polydeoxyribonucleotide Synthase [ATP] 4; DNA Ligase IV; Ligase IV, DNA, ATP-Dependent; Polynucleotide Ligase; DNA Repair Enzyme; DNA Joinase; EC 6.5.1.1; Sealase; LIG4S
Background	Gene Name: LIG4 NCBI Gene Entry: 3981 UniProt Entry: P49917
Application Information	Molecular Weight: Predicted, 104 kDa; observed, 103 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB11000 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human DNA Ligase IV
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of DNA ligase 4 expression in HeLa cells using anti-DNA ligase 4 antibody (Cat#4394, 1:2,000). Green, isotype control; red, DNA ligase 4.
Immunocytochemical staining of Hela cells with anti-DNA ligase 4 antibody (Cat#4394, 1:1,000). Nuclei were stained blue with DAPI; DNA ligase 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-DNA ligase 4 antibody (Cat#4394). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNA ligase 4 antibody (Cat#4394, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

LIG4
货号：

4394
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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