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Anti-PPM1G Rabbit Monoclonal Antibody #4422

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Flow cytometric analysis of PPM1G expression in HepG2 cells using anti-PPM1G antibody (Cat#4422, 1:2,000). Green, isotype control; red, PPM1G.
Immunocytochemical staining of HepG2 cells with anti-PPM1G antibody (Cat#4422, 1:1,000). Nuclei were stained blue with DAPI; PPM1G was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-PPM1G antibody (Cat#4422). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPM1G antibody (Cat#4422, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    PPM1G
  • 货号:
    4422
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-PPM1G Rabbit Monoclonal Antibody
Aliases PPM1G; Protein Phosphatase, Mg2+/Mn2+ Dependent 1G; PP2CG; Protein Phosphatase Magnesium-Dependent 1 Gamma; Protein Phosphatase 1G ; Protein Phosphatase 1C; EC 3.1.3.16; PP2C-Gamma; PP2CGAMMA; Protein Phosphatase 1G (Formerly 2C), Magnesium-Dependent, Gamma Isoform; Protein Phosphatase, Mg2+/Mn2+ Dependent, 1G; Protein Phosphatase 2C, Gamma Isoform; Protein Phosphatase 2C Isoform Gamma; PP2C, Gamma; PP2Cgamma; PPP2CG; PPM1C
Background

Gene Name: PPM1G

NCBI Gene Entry: 5496

UniProt Entry: O15355

Application Information

Molecular Weight: Predicted, 59 kDa; observed, 72 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11140

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human PPM1G
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of PPM1G expression in HepG2 cells using anti-PPM1G antibody (Cat#4422, 1:2,000). Green, isotype control; red, PPM1G.
  • Immunocytochemical staining of HepG2 cells with anti-PPM1G antibody (Cat#4422, 1:1,000). Nuclei were stained blue with DAPI; PPM1G was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
  • Western blotting analysis using anti-PPM1G antibody (Cat#4422). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPM1G antibody (Cat#4422, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of PPM1G expression in HepG2 cells using anti-PPM1G antibody (Cat#4422, 1:2,000). Green, isotype control; red, PPM1G.
Immunocytochemical staining of HepG2 cells with anti-PPM1G antibody (Cat#4422, 1:1,000). Nuclei were stained blue with DAPI; PPM1G was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-PPM1G antibody (Cat#4422). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPM1G antibody (Cat#4422, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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