Flow cytometric analysis of Ephrin B2 expression in HAP-1 cells using Ephrin B2 antibody (Cat#61355, 1:2,000). Green, isotype control; red, Ephrin B2.
Immunocytochemical staining of HAP-1 cells with anti-Ephrin B2 antibody (Cat#61355, 1:1,000). Nuclei were stained blue with DAPI; Ephrin B2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-ephrin B2 antibody (Cat#61355). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ephrin B2 antibody (Cat#61355, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-ephrin B2 antibody (Cat#61355). Ephrin B2 expression in wild-type (WT) and ephrin B2 (EFNB2) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ephrin B2 antibody (Cat#61355, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
