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Anti-PSMB10 Rabbit Monoclonal Antibody #4429

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Flow cytometric analysis of PSMB10 expression in HAP-1 cells using anti-PSMB10 antibody (Cat#4429, 1:2,000). Green, isotype control; red, PSMB10.

Immunocytochemical staining of H9c2 cells with anti-PSMB10 antibody (Cat#4429, 1:1,000). Nuclei were stained blue with DAPI; PSMB10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-PSMB10 antibody (Cat#4429). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PSMB10 antibody (Cat#4429, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

PSMB10
货号：

4429
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-PSMB10 Rabbit Monoclonal Antibody
Aliases	PSMB10; Proteasome 20S Subunit Beta 10; LMP10; MECL1; Proteasome (Prosome, Macropain) Subunit, Beta Type, 10; Multicatalytic Endopeptidase Complex Subunit MECl-1; Proteasome Subunit Beta Type-10; Low Molecular Mass Protein 10; Proteasome Subunit Beta 10; Proteasome Subunit Beta-2i; Macropain Subunit MECl-1; Proteasome MECl-1; EC 3.4.25.1; MGC1665; Beta2i; Proteasome Catalytic Subunit 2i; Proteasome Subunit Beta 7i; Proteasome Subunit Beta2i; Proteasome Subunit MECL1; Proteasome Subunit Β2i; PRAAS5; BETA2I
Background	Gene Name: PSMB10 NCBI Gene Entry: 5699 UniProt Entry: P40306
Application Information	Molecular Weight: Predicted, 29 kDa; observed, 25,29 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB11175 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PSMB10
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of PSMB10 expression in HAP-1 cells using anti-PSMB10 antibody (Cat#4429, 1:2,000). Green, isotype control; red, PSMB10.
Immunocytochemical staining of H9c2 cells with anti-PSMB10 antibody (Cat#4429, 1:1,000). Nuclei were stained blue with DAPI; PSMB10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PSMB10 antibody (Cat#4429). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PSMB10 antibody (Cat#4429, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

PSMB10
货号：

4429
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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