Anti-Phospho-PBK/TOPK (Thr9) Rabbit Monoclonal Antibody - 产品中心

Anti-Phospho-PBK/TOPK (Thr9) Rabbit Monoclonal Antibody #4433

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Flow cytometric analysis of phospho-PBK/TOPK (Thr9) expression in HepG2 cells using anti-phospho-PBK/TOPK (Thr9) antibody (Cat#4433, 1:2,000). Green, isotype control; red, phospho-PBK/TOPK (Thr9).

Immunocytochemical staining of Hela cells with anti-Phospho-PBK/TOPK (Thr9) antibody (Cat#4433, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PBK/TOPK (Thr9) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-phospho-PBK/TOPK (Thr9) antibody (Cat#4433). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-PBK/TOPK (Thr9) antibody (Cat#4433, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

PBK
货号：

4433
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Phospho-PBK/TOPK (Thr9) Rabbit Monoclonal Antibody
Aliases	PBK; PDZ Binding Kinase; TOPK; CT84; SPK; T-LAK Cell-Originated Protein Kinase; Cancer/Testis Antigen 84; Nori-3; Lymphokine-Activated Killer T-Cell-Originated Protein Kinase; Spermatogenesis-Related Protein Kinase; MAPKK-Like Protein Kinase; FLJ14385; Serine/Threonine Protein Kinase; Epididymis Luminal Protein 164; PDZ-Binding Kinase; EC 2.7.12.2; HEL164; NORI-3
Background	Gene Name: PBK NCBI Gene Entry: 55872 UniProt Entry: Q96KB5
Application Information	Molecular Weight: Predicted, 36 kDa; observed, 40 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB11195 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Phospho-PBK/TOPK (Thr9)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of phospho-PBK/TOPK (Thr9) expression in HepG2 cells using anti-phospho-PBK/TOPK (Thr9) antibody (Cat#4433, 1:2,000). Green, isotype control; red, phospho-PBK/TOPK (Thr9).
Immunocytochemical staining of Hela cells with anti-Phospho-PBK/TOPK (Thr9) antibody (Cat#4433, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PBK/TOPK (Thr9) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-PBK/TOPK (Thr9) antibody (Cat#4433). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-PBK/TOPK (Thr9) antibody (Cat#4433, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

PBK
货号：

4433
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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