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Anti-Proteasome Activator Subunit 1 Rabbit Monoclonal Antibody #4466

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Flow cytometric analysis of proteasome activator subunit 1 expression in HT-1080 cells using anti-proteasome activator subunit 1 antibody (Cat#4466, 1:2,000). Green, isotype control; red, proteasome activator subunit 1.
Immunocytochemical staining of HT-1080 cells with anti-proteasome activator subunit 1 antibody (Cat#4466, 1:1,000). Nuclei were stained blue with DAPI; Proteasome activator subunit 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-proteasome activator subunit 1 antibody (Cat#4466). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-proteasome activator subunit 1 antibody (Cat#4466, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    PSME1
  • 货号:
    4466
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Proteasome Activator Subunit 1 Rabbit Monoclonal Antibody
Aliases Proteasome Activator Subunit 1; PA28alpha; IFI5111; Proteasome (Prosome, Macropain) Activator Subunit 1 (PA28 Alpha); Activator Of Multicatalytic Protease Subunit 1; Interferon Gamma Up-Regulated I-5111 Protein; Proteasome Activator Complex Subunit 1; 11S Regulator Complex Subunit Alpha; IGUP I-5111; Epididymis Secretory Sperm Binding Protein Li 129m; Interferon-Gamma-Inducible Protein 5111; Proteasome Activator 28 Subunit Alpha; Interferon-Gamma IEF SSP 5111; 29-KD MCP Activator Subunit; HEL-S-129m; REG-Alpha; REGalpha; PA28A; PA28a
Background

Gene Name: PSME1

NCBI Gene Entry: 5720

UniProt Entry: Q06323

Application Information

Molecular Weight: Predicted, 29 kDa; observed, 29 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11360

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human PSME1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of proteasome activator subunit 1 expression in HT-1080 cells using anti-proteasome activator subunit 1 antibody (Cat#4466, 1:2,000). Green, isotype control; red, proteasome activator subunit 1.
  • Immunocytochemical staining of HT-1080 cells with anti-proteasome activator subunit 1 antibody (Cat#4466, 1:1,000). Nuclei were stained blue with DAPI; Proteasome activator subunit 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-proteasome activator subunit 1 antibody (Cat#4466). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-proteasome activator subunit 1 antibody (Cat#4466, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of proteasome activator subunit 1 expression in HT-1080 cells using anti-proteasome activator subunit 1 antibody (Cat#4466, 1:2,000). Green, isotype control; red, proteasome activator subunit 1.
Immunocytochemical staining of HT-1080 cells with anti-proteasome activator subunit 1 antibody (Cat#4466, 1:1,000). Nuclei were stained blue with DAPI; Proteasome activator subunit 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-proteasome activator subunit 1 antibody (Cat#4466). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-proteasome activator subunit 1 antibody (Cat#4466, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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